Medicina: Ematologia: Talassemie e trapianto del midollo osseo
| English |
| mixed lymphocyte culture |
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| Attestation |
3
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| Part of speech |
Noun syntagm
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| Grammatical label |
Countable
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| Definition |
The traditional method widely used as a functional test for alloreactivity between patient and donor is mixed lymphocyte culture (MLC). However, this method is unable to determine either the number or the functional capacity of responding T lymphocytes and has proved insufficiently sensitive to predict the incidence and severity of GVHD after HLA-identical sibling transplantation. In mixed lymphocyte culture (MLC) lymphocytes are collected from the lymph nodes (for responder cells) and the spleen (for stimulator cells) of rats with and without transplants by centrifugation. After the lymphocytes are washed with culture medium, stimulator cells were treated with mitomycin C. The tissue culture medium (RPMI 1640, GIBCO, Grand Island, N.Y.) used in this experiment was supplemented with 2-mercaptoethanol, heat-inactivated fetal calf serum, L-glutamine and penicillin-streptomycin.
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| Definition source |
Dickinson, AM., et al. (1998). ‘Predicting graft-versus-host disease in HLA identical bone marrow transplants’. Transplantation 66(7):857. (RISCEN05)
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| Context |
Bone marrow cells (BMCs) from LEW rats with LSGs showed strong, nonspecific, suppressive effects on the proliferative response in the mixed lymphocyte culture reaction, suggesting that one of the possible explanations for tolerance might be the involvement of a suppressor mechanism.
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| Context source |
Matsuura, T., et al. (1997). ‘Suppressed alloreactivity and mixed chimerism in rats with accepted cardiac allografts by intrathymic injection of donor bone marrow cells’. Transplant International 10(4):262-7. (RISCEN16)
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| Subject field |
Haemopoiesis
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| Related concept |
Conditioning regimen, bone marrow, related donor, HLA-identical donor, HLA-compatible donor, unrelated donor, allogeneic bone marrow transplantation, autologous bone marrow transplantation, hematopoietic stem cell transplantation
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| it |
Coltura mista linfocitaria
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| Reliability code |
3
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