Medicina: Oncoematologia pediatrica: Leucemia linfoblastica acuta
| English |
| cytochemistry |
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| Attestation |
3
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| Definition |
Identification of the chemical components of cells - is conducted to distinguish different types of leukemia. Cytochemical tests often use special colored dyes (stains) that are only visible under a microscope. For example, one stain turns the granules of most acute myelogenous leukemia (AML) cells black, although acute lymphocytic leukemia (ALL) cells are unaffected by this substance. Leukocyte alkaline phosphate (LAP) or neutrophil alkaline phophatase (NAP) tests formerly have been used to distinguish CML from other types of leukemia and noncancerous blood disorders; however, these assays no longer are considered particularly helpful in diagnosis, except in the absence of cytogenetic (cell genetic material) or other studies. Immunocytochemistry involves the use of labelled antibodies as part of the staining procedure.
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| Definition source |
http://www.oncologychannel.com/leukemias/diagnosis.shtml ;http://www.graylab.ac.uk/cgi-bin/omd?cytochemistry
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| Context |
The diagnosis was established when at least 25% lymphoblasts were present in the bone marrow (BM) or when blasts were present in the PB or cerebrospinal fluid (CSF); BM and blood smears and CSF cytospin preparations were stained by using a modified Wright staining technique and cytochemistry reactions (periodic acid-Schiff reaction, acid phosphatase, -naphthyl acetate esterase, and myeloperoxidase reaction) and were reviewed in the central laboratory of the study center by using the French-American-British criteria.
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| Context source |
en07
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| Synonym |
Immunocytochemistry
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| Subject field |
Pediatric Oncohematology
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| de |
Zytochemie
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| it |
citochimica
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| Reliability code |
3
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