Medicina: Oncoematologia pediatrica: Leucemia linfoblastica acuta
| English |
| immunocytochemistry |
  |
|
| Attestation |
3
|
| Synonym of |
cytochemistry
|
| Definition |
Techniques for staining cells or tissues using antibodies against the appropriate antigen. Although in principle the first antibody could be labelled, it is more common (and improves the visualisation) to use a second antibody directed against the first (an antiIgG). This second antibody is conjugated either with fluorochromes or appropriate enzymes for colourimetric reactions or gold beads (for electron microscopy) or with the biotin avidin system, so that the location of the primary antibody and thus the antigen, can be recognised.
|
| Definition source |
http://www.graylab.ac.uk/cgi-bin/omd?immunocytochemistry
|
| Context |
Immunocytochemistry, like flow cytometry, uses antibodies to treat the bone marrow or biopsy samples. Yet unlike flow cytometry, computers and lasers are not needed for this procedure. Instead, the sample is prepared so that specific types of cells undergo a color change that can be identified under a microscope. Immunocytochemistry allows the pathologist to identify specific types of leukemia .
|
| Context source |
http://www.oncologychannel.com/leukemias/diagnosis.shtml
|
| Subject field |
Pediatric Oncohematology
|
|
